In Vitro Complement-Binding on Cytoplasmic Structures in Normal Human Skin: I. Immunofluorescence Studies

Incubation of cryostat sections of normal human skin with normal human serum (NHS) at 37°C followed by fluorescein isothiocyanate labeled rabbit antihuman C3 (FITC-R/Hu-C3) yields cytoplasmic staining of various cell types including keratinocytes of the upper epidermal layers, melanocytes, fibroblasts, smooth muscle cells, and cells lining vascular structures.Deposition of C3 on the respective cytoplasmic structures is most likely due to activation of the classical complement (C) cascade on these structures since no fluorescent staining is observed when serum of patients with hereditary C4-deficiency is used instead of NHS or when incubation with NHS is performed in the presence of EDTA or EGTA in concentrations known to inhibit classical C pathway activation. Further evidence suggesting the involvement of the classical C pathway comes from the finding that incubation of cryostat skin sections with NHS followed by FITC labeled rabbit antihuman Clq (FITC-R/Hu-Clq) results in a fluorescent staining pattern remarkably similar to that seen after exposure of cryostat skin sections to NHS and FITC-R/ Hu-C3.Although formal proof is lacking, our investigations strongly indicate that binding to and activation of C components on cytoplasmic structures occur independently of the presence of circulating antibodies. This assumption is based on the finding that in 17 out of 20 NHS we were not able to detect any skin reactive antibodies by indirect immunofluorescence (IF) techniques. More conclusive evidence for a direct, antibody-independent interaction between C components and cytoplasmic structures is provided by the observation that incubation of the substrate with purified Clq followed by FITC-R/ Hu-Clq results in cytoplasmic staining of some of the skin cell populations described above.The phenomenon of C-binding adn activation on cytoplasmic structures of normal human skin cells may be a critical event in the initiation of complement mediated pathopysiological reactions of the skin

Three-Nucleon Force and the Δ\Delta-Mechanism for Pion Production and Pion Absorption

The description of the three-nucleon system in terms of nucleon and $\Delta$ degrees of freedom is extended to allow for explicit pion production (absorption) from single dynamic $\Delta$ de-excitation (excitation) processes. This mechanism yields an energy dependent effective three-body hamiltonean. The Faddeev equations for the trinucleon bound state are solved with a force model that has already been tested in the two-nucleon system above pion-production threshold. The binding energy and other bound state properties are calculated. The contribution to the effective three-nucleon force arising from the pionic degrees of freedom is evaluated. The validity of previous coupled-channel calculations with explicit but stable $\Delta$ isobar components in the wavefunction is studied.Comment: 23 pages in Revtex 3.0, 9 figures (not included, available as postscript files upon request), CEBAF-TH-93-0

Geometry: The leading parameter for the Poisson’s ratio of bending-dominated cellular solids

Control over the deformation behaviour that a cellular structure shows in response to imposed external forces is a requirement for the effective design of mechanical metamaterials, in particular those with negative Poisson’s ratio. This article sheds light on the old question of the relationship between geometric microstructure and mechanical response, by comparison of the deformation properties of bar-and-joint-frameworks with those of their realisation as a cellular solid made from linear-elastic material. For ordered planar tessellation models, we find a classification in terms of the number of degrees of freedom of the framework model: first, in cases where the geometry uniquely prescribes a single deformation mode of the framework model, the mechanical deformation and Poisson’s ratio of the linearly-elastic cellular solid closely follow those of the unique deformation mode; the result is a bending-dominated deformation with negligible dependence of the effective Poisson’s ratio on the underlying material’s Poisson’s ratio and small values of the effective Young’s modulus. Second, in the case of rigid structures or when geometric degeneracy prevents the bending-dominated deformation mode, the effective Poisson’s ratio is material-dependent and the Young’s modulus View the MathML sourceE˜cs large. All analysed structures of this type have positive values of the Poisson’s ratio and large values of View the MathML sourceE˜cs. Third, in the case, where the framework has multiple deformation modes, geometry alone does not suffice to determine the mechanical deformation. These results clarify the relationship between mechanical properties of a linear-elastic cellular solid and its corresponding bar-and-joint framework abstraction. They also raise the question if, in essence, auxetic behaviour is restricted to the geometry-guided class of bending-dominated structures corresponding to unique mechanisms, with inherently low values of the Young’s modulus

Persistent detwinning of iron pnictides by small magnetic fields

Our comprehensive study on EuFe$_2$As$_2$ reveals a dramatic reduction of magnetic detwinning fields compared to other AFe$_2$As$_2$ (A = Ba, Sr, Ca) iron pnictides by indirect magneto-elastic coupling of the Eu$^{2+}$ ions. We find that only 0.1T are sufficient for persistent detwinning below the local Eu$^{2+}$ ordering; above $T_\text{Eu}$ = 19K, higher fields are necessary. Even after the field is switched off, a significant imbalance of twin domains remains constant up to the structural and electronic phase transition (190K). This persistent detwinning provides the unique possibility to study the low temperature electronic in-plane anisotropy of iron pnictides without applying any symmetrybreaking external force.Comment: accepted by Physical Review Letter

Do biometric parameters improve the quality of optic nerve head measurements with spectral domain optical coherence tomography?

Funding Information: A part of the study data have been presented as a free paper oral presentation ‘ Clinical quality assessment of optic nerve head measurements with spectral domain optical coherence tomography—preliminary results’ in 118. Congress of the DOG (German Society of Ophthalmology) 2020. Publisher Copyright: © 2022, The Author(s).Background: Spectral domain optical coherence tomography (SD-OCT) is a widely applied non-invasive technique for evaluating optic nerve head parameters. The aim of this study was to evaluate the impact of biometric parameters such as the spherical equivalent (SE) and the anterior corneal curvature (ACC) on the peripapillary retinal nerve fiber layer (pRNFL), Bruch’s membrane opening (BMO), and the minimum rim width (MRW) measurements performed by spectral domain optical coherence tomography (SD-OCT) in glaucomatous and healthy eyes. Methods: In this cross-sectional, case–control prospective pilot study, the glaucoma group consisted of 50 patients with previously diagnosed and treated glaucoma and one healthy group of 50 subjects. Two consecutive examinations of pRNFL, BMO, and MRW with SD-OCT for every patient were performed without ACC and objective refraction (imaging 1) and with them (imaging 2). Results: The interclass correlation coefficient (ICC) reflected high agreement between imaging 1 and imaging 2 in both groups. The ICC in the glaucoma and healthy groups for pRNFL (0.99 vs. 0.98), BMO (0.95 vs. 0.97), and MRW (1.0 vs. 1.0) was comparable. Conclusions: Our preliminary data from a small number of eyes showed that the measurements of pRNFL, MRW, and BMO reflected high agreement between both imaging techniques with ACC and objective refraction and without these parameters in subjects with a refractive error up to ± 6.0 diopters. Further studies with participants with higher refractive error are necessary to evaluate the impact of biometric parameters such as SE and ACC on measurements with SD-OCT.publishersversionPeer reviewe

The pion-three-nucleon problem with two-cluster connected-kernel equations

It is found that the coupled piNNN-NNN system breaks into fragments in a nontrivial way. Assuming the particles as distinguishable, there are indeed four modes of fragmentation into two clusters, while in the standard three-body problem there are three possible two-cluster partitions and conversely the four-body problem has seven different possibilities. It is shown how to formulate the pion-three-nucleon collision problem through the integral-equation approach by taking into account the proper fragmentation of the system. The final result does not depend on the assumption of separability of the two-body t-matrices. Then, the quasiparticle method a' la Grassberger-Sandhas is applied and effective two-cluster connected-kernel equations are obtained. The corresponding bound-state problem is also formulated, and the resulting homogeneous equation provides a new approach which generalizes the commonly used techniques to describe the three-nucleon bound-state problem, where the meson degrees of freedom are usually suppressed.Comment: 20 pages, REVTeX, with 3 COLOR figures (PostScript

Label-free peptide profiling of Orbitrap™ full mass spectra

Background. We developed a new version of the open source software package Peptrix that can yet compare large numbers of Orbitrap™ LC-MS data. The peptide profiling results for Peptrix on MS1 spectra were compared with those obtained from a small selection of open source and commercial software packages: msInspect, Sieve™ and Progenesis™. The properties compared in these packages were speed, total number of detected masses, redundancy of masses, reproducibility in numbers and CV of intensity, overlap of masses, and differences in peptide peak intensities. Reproducibility measurements were taken for the different MS1 software applications by measuring in triplicate a complex peptide mixture of immunoglobulin on the Orbitrap™ mass spectrometer. Values of peptide masses detected from the high intensity peaks of the MS1 spectra by peptide profiling were verified with values of the MS2 fragmented and sequenced masses that resulted in protein identifications with a significant score. Findings. Peptrix finds about the same number of peptide features as the other packages, but peptide masses are in some cases approximately 5 to 10 times less redundant present in the peptide profile matrix. The Peptrix profile matrix displays the largest overlap when comparing the number of masses in a pair between two software applications. The overlap of peptide masses between software packages of low intensity peaks in the spectra is remarkably low with about 50% of the detected masses in the individual packages. Peptrix does not differ from the other packages in detecting 96% of the masses that relate to highly abundant sequenced proteins. MS1 peak intensities vary between the applications in a non linear way as they are not processed using the same method. Conclusions. Peptrix is capable of peptide profiling using Orbitrap™ files and finding differential expressed peptides in body fluid and tissue samples. The number of peptide masses detected in Orbitrap™ files can be increased by using more MS1 peptide profiling applications, including Peptrix, since it appears from the comparison of Peptrix with the other applications that all software packages have likely a high false negative rate of low intensity peptide peaks (missing peptides)

Infrared behavior of the gluon propagator in lattice Landau gauge: the three-dimensional case

We evaluate numerically the three-momentum-space gluon propagator in the lattice Landau gauge, for three-dimensional pure-SU(2) lattice gauge theory with periodic boundary conditions. Simulations are done for nine different values of the coupling $\beta$, from $\beta = 0$ (strong coupling) to $\beta = 6.0$ (in the scaling region), and for lattice sizes up to $V = 64^3$. In the limit of large lattice volume we observe, in all cases, a gluon propagator decreasing for momenta smaller than a constant value $p_{dec}$. From our data we estimate $p_{dec} \approx 350$ MeV. The result of a gluon propagator decreasing in the infrared limit has a straightforward interpretation as resulting from the proximity of the so-called first Gribov horizon in the infrared directions.Comment: 14 pages, BI-TP 99/03 preprint, correction in the Acknowledgments section. To appear in Phys.Rev.

Comparative Genomics of the Genus Methanohalophilus, Including a Newly Isolated Strain From Kebrit Deep in the Red Sea

Halophilic methanogens play an important role in the carbon cycle in hypersaline environments, but are under-represented in culture collections. In this study, we describe a novel Methanohalophilus strain that was isolated from the sulfide-rich brine-seawater interface of Kebrit Deep in the Red Sea. Based on physiological and phylogenomic features, strain RSK, which is the first methanogenic archaeon to be isolated from a deep hypersaline anoxic brine lake of the Red Sea, represents a novel species of this genus. In order to compare the genetic traits underpinning the adaptations of this genus in diverse hypersaline environments, we sequenced the genome of strain RSK and compared it with genomes of previously isolated and well characterized species in this genus (Methanohalophilus mahii, Methanohalophilus halophilus, Methanohalophilus portucalensis, and Methanohalophilus euhalobius). These analyses revealed a highly conserved genomic core of greater than 93% of annotated genes (1490 genes) containing pathways for methylotrophic methanogenesis, osmoprotection through salt-out strategy, and oxidative stress response, among others. Despite the high degree of genomic conservation, species-specific differences in sulfur and glycogen metabolisms, viral resistance, amino acid, and peptide uptake machineries were also evident. Thus, while Methanohalophilus species are found in diverse extreme environments, each genotype also possesses adaptive traits that are likely relevant in their respective hypersaline habitats